Anti-USP8 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P40818
Gene Id	9101
Gene Symbols	USP8
Protein Name / Synonyms	USP8, HumORF8, SPG59, UBPY, ubiquitin specific peptidase 8
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a protein that belongs to the ubiquitin-specific processing protease family of proteins. The encoded protein is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage. This protein is required for the cell to enter the S phase of the cell cycle and also functions as a positive regulator in the Hedgehog signaling pathway in development. Pseudogenes of this gene are present on chromosomes 2 and 6. Alternate splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

115kDa/127kDa

Observed MW

128kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human USP8 (NP_005145.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using USP8 antibody (144-07031) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - USP8 Polyclonal Antibody (144-07031)

Immunofluorescence analysis of A-549 cells using USP8 antibody (144-07031). Blue: DAPI for nuclear staining.
Immunofluorescence - USP8 Polyclonal Antibody (144-07031)

Expiration:

12 months from the date of shipment when stored properly.

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