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Anti-UVRAG Antibody
Catalog #: 144-08462
UVRAG Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9P2Y5 |
| Gene Id | 7405 |
| Gene Symbols | UVRAG |
| Protein Name / Synonyms | UVRAG, DHTX, VPS38, p63, UV radiation resistance associated |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene complements the ultraviolet sensitivity of xeroderma pigmentosum group C cells and encodes a protein with a C2 domain. The protein activates the Beclin1-PI(3)KC3 complex, promoting autophagy and suppressing the proliferation and tumorigenicity of human colon cancer cells. Chromosomal aberrations involving this gene are associated with left-right axis malformation and mutations in this gene have been associated with colon cancer.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
35kDa/78kDa
Observed MW
100kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 501-699 of human UVRAG (NP_003360.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using UVRAG antibody (144-08462) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human liver injury using UVRAG antibody (144-08462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using UVRAG antibody (144-08462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using UVRAG antibody (144-08462) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.