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Anti-VANGL2 Antibody

Catalog #: 144-07825

VANGL2 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
Q9ULK5
Gene Id
57216
Gene Symbols
VANGL2
Protein Name / Synonyms
VANGL2, LPP1, LTAP, STB1, STBM, STBM1, vang-like protein 2
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC
Research AreaCardiovascular Disease
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene is a membrane protein involved in the regulation of planar cell polarity, especially in the stereociliary bundles of the cochlea. The encoded protein transmits directional signals to individual cells or groups of cells in epithelial sheets. This protein is also involved in the development of the neural plate.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW
59kDa
Observed MW
70kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human VANGL2 (NP_065068.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using VANGL2 antibody (144-07825) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - VANGL2 antibody (144-07825)

Immunohistochemistry of paraffin-embedded human kidney cancer using VANGL2 antibody (144-07825) at dilution of 1:100 (40x lens).
Immunohistochemistry - VANGL2 antibody (144-07825)

Expiration:
12 months from the date of shipment when stored properly.