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Anti-VAV3 Antibody
Catalog #: 144-61027
VAV3 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q9UKW4 |
| Gene Id | 10451 |
| Gene Symbols | VAV3 |
| Protein Name / Synonyms | VAV3, guanine nucleotide exchange factor VAV3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. This gene product acts as a GEF preferentially for RhoG, RhoA, and to a lesser extent, RAC1, and it associates maximally with the nucleotide-free states of these GTPases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Dilutions
WB 1:200 - 1:2000
Format
Calculated MW
32kDa/86kDa/97kDa/100kDa
Observed MW
105kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 560-680 of human VAV3 (NP_006104.4).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using VAV3 antibody (144-015385) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 2s.
Expiration:
12 months from the date of shipment when stored properly.