Anti-VILIP3 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P37235
Gene Id	3241
Gene Symbols	HPCAL1
Protein Name / Synonyms	VILIP3 (N-term)
Clonality	Polyclonal
Preparation	This VILIP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-32 amino acids from the N-terminal region of human VILIP3.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

VILIP3 is a member of neuron-specific calcium-binding proteins family found in the retina and brain. It is highly similar to human hippocalcin protein and nearly identical to the rat and mouse hippocalcin like-1 proteins. It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation and may be of relevance for neuronal signalling in the central nervous system. There are two alternatively spliced transcript variants of this gene, with multiple polyadenylation sites. Transcript variant 1 utilizes a different exon and also lacks one exon in the 5' UTR, as compared to variant 2; thus, the encoded protein is the same.

Specificity

Other Names

HPCAL1; BDR1; Hippocalcin-like protein 1; Calcium-binding protein BDR-1; HLP2; Visinin-like protein 3

NCBI Accession #

NP_001245286.1;NP_001245287.1;NP_001245288.1;NP_002140.2;NP_602293.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

22313

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of VILIP3 (arrow) using rabbit polyclonal VILIP3 Antibody (N-term) (Cat# 102-12799). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VILIP3 gene (Lane 2) (Origene Technologies).
	The anti-VILIP3 Pab (Cat# 102-12799) is used in Western blot to detect VILIP3 in mouse cerebellum tissue lysate.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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