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Anti-Villin Antibody
Catalog #: 188-11124
Mouse anti-Human Villin Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Mouse |
| Accession Number | P09327 |
| Gene Id | 7429 |
| Gene Symbols | VIL1,VIL |
| Protein Name / Synonyms | Villin-1 |
| Immunogen | Amino acids 179-311 from the human protein were used as the immunogen for this Villin antibody. |
| Isotype | Kappa, IgG1 |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
| Recommended Applications | FACS: 0.5-1ug/10^6 cells in 0.1ml IF: 1-2ug/ml Western blot: 1-2ug/ml IHC (FFPE): 0.25-0.5ug/ml for 30 min at RT |
| Domain / Region / Terminus | Cytoplasmic and cell surface |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
Recognizes a protein of ~93kDa which is identified as Villin (VIL1). It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, Villin is predominantly expressed in tumors of colorectal origin. Antibody to Villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
Storage
Store the Villin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.