Anti-Vinculin Antibody

Product Description

Specifications

Size	200 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q64727
Gene Id	22330
Gene Symbols	Vcl
Protein Name / Synonyms	Vinculin, Metavinculin, Vcl
Clonality	Polyclonal
Clone	RB55982
Isotype	Kappa, Rabbit
Recommended Applications	FC, IHC-P, Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity).

Other Information

Antigen Type

Synthesized Peptide

Format

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Calculated Molecular Weight (Da)

116717

Antigen Source

Human

Images

FC (1:25)

Overlay histogram showing C2C12 cells stained with 102-25111(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (102-25111, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1?g/1x10

Expiration:

12 months from the date of shipment when stored properly.

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