Anti-VPS29 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9UBQ0
Gene Id	51699
Gene Symbols	VPS29
Protein Name / Synonyms	VPS29, DC15, DC7, PEP11, VPS29, retromer complex component
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene belongs to a group of vacuolar protein sorting (VPS) genes that, when functionally impaired, disrupt the efficient delivery of vacuolar hydrolases. The protein encoded by this gene is a component of a large multimeric complex, termed the retromer complex, which is involved in retrograde transport of proteins from endosomes to the trans-Golgi network. This VPS protein may be involved in the formation of the inner shell of the retromer coat for retrograde vesicles leaving the prevacuolar compartment. Alternative splice variants encoding different isoforms and representing non-protein coding transcripts have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

20kDa

Observed MW

20kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-186 of human VPS29 (NP_476528.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using VPS29 antibody (144-013098) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - VPS29 antibody (144-013098)

Expiration:

12 months from the date of shipment when stored properly.

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