Anti-VSNL1 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P62760
Gene Id	7447
Gene Symbols	VSNL1
Protein Name / Synonyms	VSNL1 (Center)
Clonality	Polyclonal
Preparation	This VSNL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 47-75 amino acids from the Central region of human VSNL1.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene is a member of the visinin/recoverin subfamily of neuronal calcium sensor proteins. The encoded protein is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium-dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.

Specificity

Predicted Reactivity

Rat, Bovine, Chicken, Monkey

Other Names

VSNL1; VISL1; Visinin-like protein 1; Hippocalcin-like protein 3

NCBI Accession #

NP_003376.2

Other Accession #

P62762; P62761; Q4R5F7; P62764; P62763; P35332; Q8BGZ1; Q9UM19; P29104; NP_003376

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

22142

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; FC: 1:10-50

	Western blot analysis of VSNL1 (arrow) using rabbit polyclonal VSNL1 Antibody (Center) (Cat# 102-11445). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the VSNL1 gene.
	VSNL1 Antibody (Center) (Cat# 102-11445) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the VSNL1 antibody detected the VSNL1 protein (arrow).
	VSNL1 Antibody (Center) (Cat# 102-11445)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of VSNL1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
	VSNL1 Antibody (Center) (Cat# 102-11445) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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