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Anti-WDR48 Antibody
Catalog #: 144-06854
WDR48 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q8TAF3 |
| Gene Id | 57599 |
| Gene Symbols | WDR48 |
| Protein Name / Synonyms | WDR48, P80, SPG60, UAF1, WD repeat domain 48 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene has been shown to interact with ubiquitin specific peptidase 1 (USP1), activating the deubiquitinating activity of USP1 and allowing it to remove the ubiquitin moiety from monoubiquitinated FANCD2. FANCD2 is ubiquitinated in response to DNA damage.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
13kDa/45kDa/66kDa/75kDa/76kDa
Observed MW
76kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 450-677 of human WDR48 (NP_065890.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using WDR48 antibody (144-06854) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat liver using WDR48 antibody (144-06854) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using WDR48 antibody (144-06854) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using WDR48 antibody (144-06854) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.