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Anti-Wee1 (Center) Antibody
Catalog #: 102-23435
Rabbit Anti-Mouse Wee1 (Center) Antibody, 400 µl
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P47810|Q63802|NP_033542.2 |
| Gene Id | 22390 |
| Gene Symbols | Wee1 |
| Protein Name / Synonyms | Wee1-like protein kinase, Wee1A kinase, Wee1 |
| Clonality | Polyclonal |
| Clone | RB39511 |
| Isotype | Kappa, Rabbit |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Wee1 may act as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur (By similarity).
Other Information
NCBI Accession #
NP_033542.2
Antigen Type
Synthetic Peptide
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Calculated Molecular Weight (Da)
71578
Antigen Source
Mouse
Images
WB (1:1000)
Mouse Wee1 Antibody (Center) (Cat. #102-23435) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the Wee1 antibody detected the Wee1 protein (arrow).
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.