Rabbit Anti-Wee1(S123) Antibody, 200 µl
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Product Description
Specifications
| Size | 200 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P30291 |
| Gene Id | 7465 |
| Gene Symbols | WEE1 |
| Protein Name / Synonyms | Wee1-like protein kinase, WEE1hu, 2.7.10.2, Wee1A kinase, WEE1 |
| Clonality | Polyclonal |
| Clone | RB57984 |
| Isotype | Kappa, Rabbit |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Other Information
Antigen Type
Synthesized Peptide
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Calculated Molecular Weight (Da)
71597
Antigen Source
Human
Images
WB (1:2000)
All lanes : Anti-Wee1(S123) Antibody at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 72 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.