Anti-WIPI1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q5MNZ9
Gene Id	55062
Gene Symbols	WIPI1
Protein Name / Synonyms	WIPI1, ATG18, ATG18A, WIPI49, WD repeat domain, phosphoinositide interacting 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a WD40 repeat protein. Members of the WD40 repeat family are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

48kDa

Observed MW

46kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 187-446 of human WIPI1 (NP_060453.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using WIPI1 antibody (144-07528) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - WIPI1 Polyclonal Antibody (144-07528)

Immunohistochemistry of paraffin-embedded human brain cancer using WIPI1 Antibody (144-07528) at dilution of 1:100 (40x lens).
Immunohistochemistry - WIPI1 Polyclonal Antibody (144-07528)

Expiration:

12 months from the date of shipment when stored properly.

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