RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

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770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-WWTR1 Antibody

Catalog #: 144-61215

WWTR1 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
Q9GZV5
Gene Id
25937
Gene Symbols
WWTR1
Protein Name / Synonyms
WWTR1, TAZ, WW domain containing transcription regulator 1
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF
Research AreaCardiovascular Disease
Shipping TypeBlue ice
Storage-20°C

Description

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW
44kDa
Observed MW
44kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human WWTR1 (NP_056287.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using WWTR1 antibody (144-015806) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - WWTR1 antibody (144-015806)

Immunofluorescence analysis of U2OS cells using WWTR1 antibody (144-015806) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - WWTR1 antibody (144-015806)

Expiration:
12 months from the date of shipment when stored properly.