Anti-XPO5 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9HAV4
Gene Id	57510
Gene Symbols	XPO5
Protein Name / Synonyms	XPO5, exp5, exportin-5
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the karyopherin family that is required for the transport of small RNAs and double-stranded RNA-binding proteins from the nucleus to the cytoplasm. The encoded protein translocates cargo through the nuclear pore complex in a RanGTP-dependent process.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:200

Format

Calculated MW

136kDa

Observed MW

125kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 925-1204 of human XPO5 (NP_065801.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using XPO5 antibody (144-06790).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - XPO5 Polyclonal Antibody (144-06790)

Immunohistochemistry of paraffin-embedded human oophoroma using XPO5 antibody (144-06790) at dilution of 1:100 (40x lens).
Immunohistochemistry - XPO5 Polyclonal Antibody (144-06790)

Immunohistochemistry of paraffin-embedded mouse jejunum using XPO5 antibody (144-06790) at dilution of 1:100 (40x lens).
Immunohistochemistry - XPO5 Polyclonal Antibody (144-06790)

Immunofluorescence analysis of MCF7 cells using XPO5 antibody (144-06790).
Immunofluorescence - XPO5 Polyclonal Antibody (144-06790)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug XPO5 antibody (144-06790). Western blot was performed from the immunoprecipitate using XPO5 antibody (144-06790) at a dilition of 1:1000.
Immunoprecipitation - XPO5 Polyclonal Antibody (144-06790)

Expiration:

12 months from the date of shipment when stored properly.

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