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Anti-XRCC3 Antibody
Catalog #: 144-02134
XRCC3 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O43542 |
| Gene Id | 7517 |
| Gene Symbols | XRCC3 |
| Protein Name / Synonyms | XRCC3, CMM6, DNA repair protein XRCC3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Alternatively spliced transcript variants encoding the same protein have been identified.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
37kDa
Observed MW
37kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 62-346 of human XRCC3 (NP_001093588.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of HL-60 cells, using XRCC3 antibody (144-02134) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat testis using XRCC3 antibody (144-02134) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using XRCC3 antibody (144-02134) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using XRCC3 antibody (144-02134) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using XRCC3 antibody (144-02134) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using XRCC3 antibody (144-02134). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.