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Anti-XRCC4 Antibody
Catalog #: 144-01677
XRCC4 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Rat |
| Host Species | Rabbit |
| Accession Number | Q13426 |
| Gene Id | 7518 |
| Gene Symbols | XRCC4 |
| Protein Name / Synonyms | XRCC4, SSMED, DNA repair protein XRCC4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternative splicing generates several transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100
IHC 1:50 - 1:200
IF 1:10 - 1:100
Format
Calculated MW
35kDa/38kDa
Observed MW
55kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-336 of human XRCC4 (NP_071801.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using XRCC4 antibody (144-01677) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human appendix using XRCC4 antibody (144-01677) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using XRCC4 antibody (144-01677) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using XRCC4 antibody (144-01677) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of 293T cells using XRCC4 antibody (144-01677) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.