3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-XRCC6 Antibody
Catalog #: 144-00883
XRCC6 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P12956 |
| Gene Id | 2547 |
| Gene Symbols | XRCC6 |
| Protein Name / Synonyms | XRCC6, CTC75, CTCBF, G22P1, KU70, ML8, TLAA, X-ray repair cross complementing 6 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
65kDa/69kDa
Observed MW
70kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 500-600 of human XRCC6 (NP_001460.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using XRCC6 antibody (144-00883) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human adenomyosis using XRCC6 Antibody (144-00883) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human brain astrocytoma using XRCC6 Antibody (144-00883) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using XRCC6 Antibody (144-00883) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using XRCC6 Antibody (144-00883) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using XRCC6 Antibody (144-00883) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using XRCC6 Antibody (144-00883) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using XRCC6 Antibody (144-00883) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendicitis using XRCC6 Antibody (144-00883) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using XRCC6 Antibody (144-00883) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using XRCC6 Antibody (144-00883) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using XRCC6 Antibody (144-00883) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using XRCC6 Antibody (144-00883) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using XRCC6 Antibody (144-00883) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using XRCC6 Antibody (144-00883) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using XRCC6 Antibody (144-00883) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using XRCC6 antibody (144-00883) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.