Anti-XRCC6 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P12956
Gene Id	2547
Gene Symbols	XRCC6
Protein Name / Synonyms	XRCC6, CTC75, CTCBF, G22P1, KU70, ML8, TLAA, X-ray repair cross complementing 6
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IP 1:20 - 1:50

Format

Calculated MW

65kDa/69kDa

Observed MW

70kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 408-609 of human XRCC6 (NP_001460.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using XRCC6 antibody (144-07330) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - XRCC6 Polyclonal Antibody (144-07330)

Immunohistochemistry of paraffin-embedded rat brain using XRCC6 antibody (144-07330) at dilution of 1:100 (40x lens).
Immunohistochemistry - XRCC6 Polyclonal Antibody (144-07330)

Immunohistochemistry of paraffin-embedded human gastric cancer using XRCC6 antibody (144-07330) at dilution of 1:100 (40x lens).
Immunohistochemistry - XRCC6 Polyclonal Antibody (144-07330)

Expiration:

12 months from the date of shipment when stored properly.

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