Anti-YAP1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P46937
Gene Id	10413
Gene Symbols	YAP1
Protein Name / Synonyms	YAP1, COB1, YAP, YAP2, YAP65, YKI, Yes associated protein 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF, IP
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50

Format

Calculated MW

36kDa/48kDa/49kDa/50kDa/52kDa/53kDa/54kDa

Observed MW

72kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 155-504 of human YAP1 (NP_001123617.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using YAP1 antibody (144-01002) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - [KO Validated] YAP1 Polyclonal Antibody (144-01002)

Western blot analysis of extracts from normal (control) and YAP1 knockout (KO) HeLa cells, using YAP1 antibody (144-01002) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - [KO Validated] YAP1 Polyclonal Antibody (144-01002)

Immunohistochemistry of paraffin-embedded rat heart using YAP1 antibody (144-01002) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] YAP1 Polyclonal Antibody (144-01002)

Immunohistochemistry of paraffin-embedded human tonsil using YAP1 antibody (144-01002) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] YAP1 Polyclonal Antibody (144-01002)

Immunohistochemistry of paraffin-embedded human lung cancer using YAP1 antibody (144-01002) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] YAP1 Polyclonal Antibody (144-01002)

Immunohistochemistry of paraffin-embedded human liver cancer using YAP1 antibody (144-01002) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] YAP1 Polyclonal Antibody (144-01002)

Immunohistochemistry of paraffin-embedded human placenta using YAP1 antibody (144-01002) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] YAP1 Polyclonal Antibody (144-01002)

Immunohistochemistry of paraffin-embedded mouse spinal cord using YAP1 antibody (144-01002) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] YAP1 Polyclonal Antibody (144-01002)

Immunofluorescence analysis of HeLa cells using YAP1 antibody (144-01002) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] YAP1 Polyclonal Antibody (144-01002)

Immunofluorescence analysis of NIH/3T3 cells using YAP1 antibody (144-01002) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] YAP1 Polyclonal Antibody (144-01002)

Immunofluorescence analysis of PC12 cells using YAP1 antibody (144-01002) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] YAP1 Polyclonal Antibody (144-01002)

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug YAP1 antibody (144-01002). Western blot was performed from the immunoprecipitate using YAP1 antibody (144-01002) at a dilition of 1:1000.
Immunoprecipitation - [KO Validated] YAP1 Polyclonal Antibody (144-01002)

Expiration:

12 months from the date of shipment when stored properly.

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