RayBiotech

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ISO 13485:2016 cGMP

Anti-YTHDC2 Antibody

Catalog #: 144-60880

YTHDC2 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q9H6S0
Gene Id
64848
Gene Symbols
YTHDC2
Protein Name / Synonyms
YTHDC2, CAHL, YTH domain containing 2
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF
Research AreaCancer
Shipping TypeBlue ice
Storage-20°C

Description

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW
160kDa
Observed MW
160kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1131-1430 of human YTHDC2 (NP_073739.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using YTHDC2 antibody (144-60880) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - YTHDC2 antibody (144-60880)

Immunofluorescence analysis of NIH/3T3 cells using YTHDC2 antibody (144-60880) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - YTHDC2 antibody (144-60880)

Expiration:
12 months from the date of shipment when stored properly.