Anti-YWHAE Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P62258
Gene Id	7531
Gene Symbols	YWHAE
Protein Name / Synonyms	YWHAE, 14-3-3E, HEL2, KCIP-1, MDCR, MDS, 14-3-3 protein epsilon
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

26kDa/29kDa

Observed MW

29kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human YWHAE (NP_006752.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using YWHAE Antibody (144-01058) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - YWHAE Polyclonal Antibody (144-01058)

Expiration:

12 months from the date of shipment when stored properly.

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