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Anti-YWHAG Antibody
Catalog #: 144-03043
YWHAG Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P61981 |
| Gene Id | 7532 |
| Gene Symbols | YWHAG |
| Protein Name / Synonyms | YWHAG, 14-3-3GAMMA, PPP1R170, 14-3-3 protein gamma |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
28kDa
Observed MW
30kDa
Immunogen
A synthetic peptide of human YWHAG
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using YWHAG antibody (144-03043) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Expiration:
12 months from the date of shipment when stored properly.