Anti-ZAP70 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P43403
Gene Id	7535
Gene Symbols	ZAP70
Protein Name / Synonyms	ZAP70 (N-term)
Clonality	Polyclonal
Preparation	This ZAP70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-130 amino acids from the N-terminal region of human ZAP70.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Inflammation, Autoimmune Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.

Specificity

Predicted Reactivity

Mouse

Other Names

ZAP70; SRK; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase

NCBI Accession #

NP_001070.2;NP_997402.1

Other Accession #

P43404; NP_997402.1; NP_001070.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

69872

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	ZAP70 Antibody (N-term) (Cat# 102-12333) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ZAP70 antibody detected the ZAP70 protein (arrow).
	ZAP70 Antibody (N-term) (102-12333)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZAP70 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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