Anti-ZFYVE9 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O95405
Gene Id	9372
Gene Symbols	ZFYVE9
Protein Name / Synonyms	ZFYVE9, MADHIP, NSP, PPP1R173, SARA, SMADIP, zinc finger FYVE-type containing 9
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a double zinc finger motif-containing protein that participates in the transforming growth factor-beta (TGFB) signalling pathway. The encoded protein interacts directly with SMAD2 and SMAD3, and recruits SMAD2 to the TGFB receptor. There are multiple pseudogenes for this gene on chromosomes 2, 15, and X. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:200 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

83kDa/150kDa/156kDa

Observed MW

156kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ZFYVE9 (NP_004790.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ZFYVE9 antibody (144-09041) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.
Western blot - ZFYVE9 antibody (144-09041)

Immunofluorescence analysis of HeLa cells using ZFYVE9 antibody (144-09041) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - ZFYVE9 antibody (144-09041)

Expiration:

12 months from the date of shipment when stored properly.

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