RayBiotech

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Anti-ZNF195 Antibody

Catalog #: 144-07347

ZNF195 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
O14628
Gene Id
7748
Gene Symbols
ZNF195
Protein Name / Synonyms
ZNF195, HRF1, ZNFP104, zinc finger protein 195
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a protein belonging to the Krueppel C2H2-type zinc-finger protein family. These family members are transcription factors that are implicated in a variety of cellular processes. This gene is located near the centromeric border of chromosome 11p15.5, next to an imprinted domain that is associated with maternal-specific loss of heterozygosity in Wilms' tumors. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW
10kDa/64kDa/69kDa/70kDa/72kDa
Observed MW
65kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ZNF195 (NP_001123991.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ZNF195 antibody (144-07347) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - ZNF195 antibody (144-07347)

Immunofluorescence analysis of A549 cells using ZNF195 antibody (144-07347). Blue: DAPI for nuclear staining.
Immunofluorescence - ZNF195 antibody (144-07347)

Expiration:
12 months from the date of shipment when stored properly.