RayBiotech

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ISO 13485:2016 cGMP

Anti-ZNF331 Antibody

Catalog #: 144-61070

ZNF331 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q9NQX6
Gene Id
55422
Gene Symbols
ZNF331
Protein Name / Synonyms
ZNF331, RITA, ZNF361, ZNF463, zinc finger protein 331
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a zinc finger protein containing a KRAB (Kruppel-associated box) domain found in transcriptional repressors. This gene may be methylated and silenced in cancer cells. This gene is located within a differentially methylated region (DMR) and shows allele-specific expression in placenta. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding the same protein.

Dilutions

WB 1:200 - 1:2000

Format

Calculated MW
53kDa
Observed MW
56kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 50-130 of human ZNF331 (NP_061025.5).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ZNF331 antibody (144-61070) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - ZNF331 antibody (144-61070)

Expiration:
12 months from the date of shipment when stored properly.