Anti-ZNF346 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9UL40
Gene Id	23567
Gene Symbols	ZNF346
Protein Name / Synonyms	ZNF346, JAZ, Zfp346, zinc finger protein 346
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a nucleolar, zinc finger protein that preferentially binds to double-stranded (ds) RNA or RNA/DNA hybrids, rather than DNA alone. Mutational studies indicate that the zinc finger domains are not only essential for dsRNA binding, but are also required for its nucleolar localization. The encoded protein may be involved in cell growth and survival. It plays a role in protecting neurons by inhibiting cell cycle re-entry via stimulation of p21 gene expression. Alternative splicing of this gene results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

29kDa/32kDa/35kDa

Observed MW

33kDa, 38kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-294 of human ZNF346 (NP_036411.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ZNF346 antibody (144-07507) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - ZNF346 antibody (144-07507)

Immunohistochemistry of paraffin-embedded rat brain using ZNF346 antibody (144-07507) at dilution of 1:100 (40x lens).
Immunohistochemistry - ZNF346 antibody (144-07507)

Immunofluorescence analysis of A549 cells using ZNF346 antibody (144-07507). Blue: DAPI for nuclear staining.
Immunofluorescence - ZNF346 antibody (144-07507)

Expiration:

12 months from the date of shipment when stored properly.

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