Aspartate aminotransferase (AST), also known as serum glutamic-oxaloacetic transaminase (GOT, SGOT), is an enzyme that catalyzes the transfer of an alpha amino group from aspartate to α-ketoglutarate, producing glutamate and oxaloacetic acid. The reaction is important in both amino acid biosynthesis and degradation. Elevated levels of AST in the blood are often indicative of liver or muscle diseases. AST is commonly measured as part of diagnostic tests for liver function, myocardial infarction, acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease, and trauma.

Aspartate Aminotransferase (AST) Activity Assay Kit offers a user-friendly, reliable, and highly sensitive method for quantifying AST activity in plasma, serum, cell lysates, and other biological fluid samples. In this assay, AST catalyzes the transfer of the amino group from L-aspartate to α-Ketoglutarate, generating oxalacetate and L-glutamate. Oxalacetate then undergoes reduction with simultaneous oxidation of NADH to NAD in the malate dehydrogenase (MDH) catalyzed indicator reaction. The resulting rate of decrease in absorbance at 340nm is directly proportional to the AST activity.

• Microplate reader capable of measuring absorbance at 340 nm at 37°C
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Multi-channel pipettes to deliver 20 μL to 200 μL volumes
• Tubes to prepare sample dilutions
• Incubator at 37°C
• 15 ml conical tubes

1. Set up a microplate reader or a microplate incubator at 37°C.
2. Prepare Working Solution, and incubate it at 37°C for at least five minutes.
3. Prepare Positive Control.
4. Pipette 10 μL of sample, Sample Buffer (as the blank) or Positive Control into each well of the 96-well plate.
5. Transfer 100 μL of pre-warmed Working Solution into each well (it is recommended to use a multi-channel pipette), mix and incubate at 37°C for one minute.
6. After one minute, read and record absorbance at 340nm (A1 Reading). Repeat the readings every minute for the next four minutes at 37°C (A2, A3, A4, A5 Reading).