Introduction
The RayBio® ATP Quantification Assay is a rapid, sensitive, and simple method that utilizes the ATP dependency of firefly luciferase to quantify ATP content in solution via the resulting luminosity. This kit can be used to assess cell viability, cytotoxicity, and/or metabolic activity in a variety of media (cell culture, PBS, water, etc.) and can quantify as little as 0.064 picomoles in a 100 µL reaction.
Product Features
- Rapid and Sensitive
- Versatile Applications
- High Sensitivity
- Suitable for various cell types and environmental samples
Kit Components
- Dilution Buffer
- Assay Buffer
- Lysis Buffer
- ATP Standard
- Luciferase
- DTT
- D-Luciferin
- 96-Well White-Walled Microplate
- Plate Seal
Additional Materials Required
- Sterile Phosphate Buffered Saline (PBS)
- Vortex Mixer
- Tabletop centrifuge
- Pipettes and nuclease-free pipette tips (barrier tips recommended)
- Nuclease-free 15 mL conical tubes and 1.5 mL microcentrifuge tubes
- Plate reader capable of measuring luminosity
Assay Procedure Summary
- Prepare all reagents, samples and standards as instructed.
- Add standard or sample to each well of the microplate.
- Add Master Mix to each well and mix.
- Protect from light and allow to equilibrate to room temperature for 10 minutes.
- Measure luminosity.
Storage/Stability
Remove D-luciferin vials from kit and store at or below -70°C protected from light. The remaining items of the kit can be stored together at -20°C or separately according to indicated storage temperatures. Kit is stable for up to four months.
