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ISO 13485:2016 cGMP
Beta Lactamase Recombinant
Catalog #: 228-11458
Product Description
Specifications
| Size | 10 mg, 50 mg |
| Estimated Lead Time | 2-3 weeks |
| Protein Name / Synonyms | b-Lactamase, EC 3.5.2.6, TEM-1. |
| Expressed Region | MHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW. |
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
| Format | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a concentrated solution in 100mM Tris, pH7.0. |
| Activity | One unit will hydrolyze 1.0 µmole of benzyl penicillin at pH 7.0 at 25°C, in presence of EDTA. |
| Source | E. coli |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Recombinant E.coli Beta-Lactamase produced in E.Coli is a single, non-glycosylated polypeptide chain containing 264 amino acids and having a molecular mass of approximately 28.9 kDa. Beta Lactamase is purified by proprietary chromatographic techniques
More Information
Solubility
It is recommended to reconstitute the lyophilized Beta Lactamase in sterile 18MΩ-cm H2 O at a concentration of 100 µg/ml, which can then be further diluted to other aqueous solutions. The Beta Lactamase should be used in pH 7.0 - 8.0 and in temperature not higher then 45°C.
Storage/Stability
Lyophilized Beta Lactamase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Beta Lactamase Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.