Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information |
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CD40 Ligand
(TNFSF5)
- Gene Symbol: CD40LG CD40L TNFSF5
- Gene ID: 282387
- Accession #: P51749
- ELISA Kit: RayBio
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IL-1 beta
(IL-1 F2)
- Gene Symbol: IL1B
- Gene ID: 281251
- Accession #: P09428
- ELISA Kit: RayBio
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IL-10
- Gene Symbol: IL10
- Gene ID: 281246
- Accession #: P43480
- ELISA Kit: RayBio
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IL-18
- Gene Symbol: IL18 IGIF
- Gene ID: 281249
- Accession #: Q9TU73
- ELISA Kit: RayBio
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LIF
- Gene Symbol: LIF
- Gene ID: 280840
- Accession #: Q27956
- ELISA Kit: RayBio
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Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Bovine Cytokine Array Q30 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- 30 ml Centrifuge Tube
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser ScannersDon't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays!
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Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.