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Cardiac Troponin I, Human Recombinant
Catalog #: 228-11568
Product Description
Specifications
| Size | 50 µg, 1000 µg |
| Estimated Lead Time | 2-3 weeks |
| Species | Human |
| Accession Number | P19429 |
| Gene Id | 7137 |
| Protein Name / Synonyms | Troponin I cardiac muscle, Cardiac troponin I, TNNI3, TNNC1, CMH7, RCM1, cTnI, CMD2A, MGC116817. |
| Expressed Region | MADGSSDAAREPRPAPAPIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQ
IAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEE
RYDIEAKVTKNITEIADLTQKIFDLRGKFKRPTLRRVRISADAMMQALLGARAKE
SLDLRAHLKQVKKEDTEKENREVGDWRKNIDALSGMEGRKKKFES |
| Expression System | E. coli |
| Purity | > 95.0% |
| Purity Determined By | SDS-PAGE |
| Format | Sterile Filtered White lyophilized (freeze-dried) powder |
| Formulation | Human TNNI3 was lyophilized in 0.01M HCl |
| Reconstitution | It is recommended to reconstitute the lyophilized TNNI3 with urea /Tris buffer (20mM Tris, pH 7.5, 5 mM EDTA, 7 M urea and 15 mM 2-mercaptoethanol) not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Storage/Stability
Lyophilized TNNI3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Cardiac Troponin I should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Description
Recombinant Human TNNI3 produced in E. coli is a single, non-glycosylated, polypeptide chain containing 210 amino acids having an additional methionine residue at N-terminus. The TNNI3 is purified by proprietary chromatographic techniques.