Rabbit Anti-Human CDC20 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q12834 |
| Gene Id | 991 |
| Gene Symbols | CDC20 |
| Protein Name / Synonyms | CDC20 (N-term) |
| Clonality | Polyclonal |
| Preparation | This CDC20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-84 amino acids from the N-terminal region of human CDC20. |
| Concentration (lot specific) | 0.400 mg/ml |
| Recommended Applications | Western Blotting, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
CDC20 is an activating regulatory factor for the APC/C (anaphase promoting complex/cyclosome). It activates the ubiquitination activity of the APC/C. CDC20 confers a strict destruction-box (D-box) dependence on APC. Levels of CDC20, as well as its binding to APC, peak in mitosis and decrease drastically at early G1.
Specificity
Predicted Reactivity
Pig
Other Names
CDC20; Cell division cycle protein 20 homolog; p55CDC
NCBI Accession #
NP_001246.2
Other Accession #
Q5H7C0
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
54723
Recommended Dilutions
WB: 1:1000; FC: 1:10-50
 | Western blot analysis of CDC20 Antibody (N-term) (Cat# 102-18160) in Hela cell line lysates (35ug/lane). CDC20 (arrow) was detected using the purified Pab. |
 | CDC20 Antibody (N-term) (Cat# 102-18160) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Western blot analysis of CDC20 (arrow) using rabbit polyclonal CDC20 Antibody (N-term) (Cat# 102-18160). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDC20 gene. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.