ELISA (recommended work dilution= 1: 1,000-5,000), Western Blotting (recommended work dilution= 1: 1,000-2,000)
Immunogen was synthetic peptide derived from Human GDF5. This antibody was produced from a chicken immunized with the immunogen. The IgY fraction was purified from collected egg followed by in-house affinity chromatography.
Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial.The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can be stored at 4°C for 1 month. Please avoid freeze-thaw cycles.
Growth/differentiation factor 5 (GDF5) is a protein that in humans is encoded by the GDF5 gene. The protein encoded by this gene is closely related to the bone morphogenetic protein (BMP) family and is a member of the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, HunterThompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development
The chicken anti-Human GDF5 antibody specifically detected target derived from human recombinant protein as well as selected cell lysates. Cross reactivity with mouse and rat GDF5 has not yet been tested.
Store at -20°C, if not intended for use within a month, keep it at 4°C and minimize freezing and thawing when use.
- HÃ¶tten G, Neidhardt H, Jacobowsky B, Pohl J (1994). "Cloning and expression of recombinant human growth/differentiation factor 5.". Biochem. Biophys. Res. Commun. 204 (2): 64652
- Nishitoh H, Ichijo H, Kimura M, et al. (1996). "Identification of type I and type II serine/threonine kinase receptors for growth/differentiation factor-5.". J. Biol. Chem. 271 (35): 2134552.