ELISA (recommended work dilution= 1: 32,000)
The immunogen was a synthetic peptide. This antibody was produced from a rabbit immunized with this peptide conjugated with KLH. The IgG fraction was purified from rabbit serum by Protein G affinity chromatography.
Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile 1 x PBS to a final concentration of 1 mg/ml.
This gene encodes a multi-domain secreted protein that may have a critical role in ocular and limb development. Mutations in this gene are associated with microphthalmia and limb anomalies. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
This antibody can specifically bind to human SMOC1. Cross reactivity with mouse or rat SMOC1 was no tested.
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 1 month at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.
- Vannahme, C. et al. (2002) Characterization of SMOC-1, a novel modular calcium-binding protein in basement membranes. J Biol Chem. 277(41):37977-86. Epub 2002 Jul 18