This ELISA kit shows no cross-reactivity with the following cytokines tested: Chicken Caronte, IFN gamma, IL-6, IL-10, IL-16, IL-21, Netrin-2, Pentraxin 3, RANTES (CCL5).
Accession Number
A0A1L1U0X1
Quantitative/Semi-Quantitative
Quantitative
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum
Solid Support
96-well Microplate
Method Of Detection
Colorimetric
Design Principle
Sandwich-based
Sensitivity
9 pg/ml
Detection Range
9 pg/ml - 2000 pg/ml
Recommended Dilution (Serum/Plasma)
2 fold
Estimated Lead Time
1-2 business days
Risk-Free Guarantee
We offer a 100% guarantee on all ELISA Kits or C-Series Membrane Array.
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
