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Collagen Type IV Alpha 3, Human Recombinant
Catalog #: 228-10263
Product Description
Specifications
| Size | 10 µg, 1000 µg |
| Estimated Lead Time | 2-3 weeks |
| Species | Human |
| Accession Number | Q01955 |
| Gene Id | 1285 |
| Protein Name / Synonyms | Collagen alpha-3(IV) chain, Goodpasture antigen, COL4A3, Glomerular Basal Membrane, GBM. |
| Expression System | Sf9 Insect Cells |
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
| Format | Sterile Filtered clear solution. |
| Formulation | The protein solution contains 20mM Hepes, pH 8.0, and 4M Urea |
| Recommended Applications | Direct ELISA (coating concentration 0.12-0.5 ug/ml depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination. It has been reported that the immunodominant epitope of COL4A3 is a cryptic epitope that is not easily, Direct ELISA (coating concentration 0.12-0.5 ug/ml depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination. It has been reported that the immunodominant epitope of COL4A3 is a cryptic epitope that is not easily accessible to the corresponding autoantibodies. It is necessary to treat the protein under nonreducing conditions with a denaturant such as urea to unmask the epitopes (see Hellmark et al. in Autoantibodies, Peter, J.B. and Shoenfeld, Y., eds., Elsevier B.V., 1996, pp 291-298). |
| Research Area | Angiogenesis, Autoimmune Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Description
Human a3 chain of collagen IV; identical with the antigen called "glomerular basal membrane antigen" (GBM). Recombinant antigen for solid (ELISA) and fluid phase diagnostic assays. Binds IgG-type human auto-antibodies. Calculated Molecular weight: 53 kDa. Calculated isoelectric point: pH 8.9. cDNA coding for a minicollagen version of the human collagen IV a3 chain fused to a hexa-histidine purification tag. The term minicollagen designates the removal of most of the epitope-less triplehelical collagenous region (situated between the N-terminal 7S domain and the C-terminal noncollagenous NC1 domain), which is a requirement for recombinant production of this antigen.
Immunological Functions
- Binds IgG-type human auto-antibodies.
- Standard ELISA test (checkerboard analysis of positive/negative samples.
- Immunodot test with positive/negative samples.
Stability & Storage
Storage/Stability
Recommendations for storage buffer: Ionic strength between 50 and 100 mM, neutral to slightly alkaline pH and 4 M urea as dissociating agent. Storage temperature is -70° to -80° C.Please prevent freeze-thaw cycles.