Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	1-2 business days
Species	Human
Specificity	This indirect ELISA kit shows cross-reactivity with other SARS-CoV-2 Spike variants.
Compatible Sample Types	Serum
Gene Symbols	IGG
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	96-well Microplate
Design Principle	Indirect
Method Of Detection	Colorimetric
Research Area	Infectious Disease, COVID-19
Shipping Type	Dry ice (additional shipping fee)
Storage	-80°C

Application Notes

Kit Components

SARS-CoV-2 Delta spike variant protein coated 96-well microplate
Wash Buffer
Positive Control
Biotinylated Anti-Human IgG
HRP-Streptavidin concentrate
TMB One-Step Substrate
Stop Solution
Assay Diluent
5X Sample Diluent

Other Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
100 ml and 1 liter graduated cylinders
Absorbent paper
Distilled or deionized water

Protocol Outline

Prepare all reagents, samples and standards as instructed.
Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
Add 100 μl prepared Biotinylated Anti-Human IgG Antibody into each well. Incubate 30 minutes at room temperature.
Add 100 μl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 15 minutes at room temperature.
Add 50 µl Stop Solution to each well. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20^°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80^°C.

Application Notes

Kit Components

SARS-CoV-2 Delta spike variant protein coated 96-well microplate
Wash Buffer
Positive Control
Biotinylated Anti-Human IgG
HRP-Streptavidin concentrate
TMB One-Step Substrate
Stop Solution
Assay Diluent
5X Sample Diluent

Other Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
100 ml and 1 liter graduated cylinders
Absorbent paper
Distilled or deionized water

Protocol Outline

Prepare all reagents, samples and standards as instructed.
Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
Add 100 μl prepared Biotinylated Anti-Human IgG Antibody into each well. Incubate 30 minutes at room temperature.
Add 100 μl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 15 minutes at room temperature.
Add 50 µl Stop Solution to each well. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20^°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80^°C.

Featured Products

COVID-19 Delta Spike Variant Human IgG ELISA Kit

COVID-19 Delta Spike Variant Human IgG ELISA Kit

Product Description

Specifications

Application Notes

Storage/Stability

Have this product in hand?

Featured Products

COVID-19 Delta Spike Variant Human IgG ELISA Kit

Product Description

Specifications

Application Notes

Storage/Stability

Have this product in hand?

COVID-19 Delta Spike Variant Human IgG ELISA Kit

Featured Products

COVID-19 Delta Spike Variant Human IgG ELISA Kit

COVID-19 Delta Spike Variant Human IgG ELISA Kit

Product Description

Specifications

Application Notes

Storage/Stability

Documents

Have this product in hand?

Reviews

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Featured Products

COVID-19 Delta Spike Variant Human IgG ELISA Kit

Product Description

Specifications

Application Notes

Storage/Stability

Documents

Have this product in hand?

Reviews

Ask A Question

COVID-19 Delta Spike Variant Human IgG ELISA Kit