RayBiotech's COVID-19 pseudovirus service enables the study of the SARS-CoV-2 virus in cell culture. This service is ideal for:
In the presence of Spike-ACE2 inhibitors, the level of luminescence will decrease.
Expresses the SARS-CoV-2 spike protein.
Serum / Plasma / Peptides / Proteins / Purified antibodies / Small molecules
Undiluted serum or plasma (100 µL) / Peptides (≥ 200 µg) / Proteins (≥ 25 µg) / Purified antibodies (≥ 20 µg) / Small molecules (≥ 1 mg)
No sample minimum. We charge our service based on how many 96-well plates are required. Therefore, regardless of whether 1 well is used or all 96 wells, the charge for the service will be the same.
We will perform 2 technical replicates unless otherwise requested.
You can choose between the ACE2-expressing cells, human A549 or monkey Vero. If you don't have a preference, we recommend the human A549 cell line.
Our service includes the following controls:
Duplicate technical replicates will be performed for all controls unless otherwise requested.
Additional negative controls with cells that do not express the ACE2 receptor, mouse NIH3T3 or ovine OFTU cells, can be added to this service upon request (additional fees apply). If you don't have a preference, we recommend the mouse NIH3T3 cell line.
You will receive two files:
A pseudovirus is a viral particle that can mimic viral entry and replicate only once. It is not considered a live virus.
Neutralization methods that use live SARS-CoV-2 require working under strict bio-containment conditions in biosafety level-3 (BSL-3) laboratories. These type of facilities are rare and expensive to operate. Using a SARS-CoV-2 pseudovirus, which can be handled at BSL2, is a more affordable, safe, and accessible option.
Our pseudovirus expresses the SARS-CoV-2 Spike protein on the surface while a plasmid encoding for luciferase is contained inside the particle. No other nucleic acid is present inside the pseudovirus. The Spike protein will bind to the ACE2 receptor, the membranes of the viral particle and cell will fuse, and the plasmid will be released into the cell where luciferase will be expressed. In the presence of a luciferase substrate, cells that have been successfully infected with the pseudovirus will luminescence. See "How It Works" figure (above).
The Spike-ACE2 interaction is a critical step to SARS-CoV-2 entry into host cells. Any molecule (e.g., peptides, antibodies, small molecules) that inhibits this protein interaction will result in reduced luminescence of the cell-of-interest compared to the positive control where no molecule is added.
Yes. However, if you have a large library of potential inhibitors or samples that you want to screen, you may want to consider using our plate-based COVID-19 Spike-ACE2 Binding Assay Kit. Hundreds to thousands of different samples can be screened rapidly and affordably. Molecules that are identified as inhibitors with the plate-based, in vitro assay can then be analyzed with our pseudovirus service. Please note that, as these are different experimental platforms, the results from the in vitro, plate-based assay may not match the results obtained with the pseudovirus service, and vice versa.