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COVID-19 Rapid Isothermal PCR Kit

25, 100, or 500 samples. No PCR instrument required! Suitable for the qualitative detection of SARS-CoV-2 virus in human throat swabs. Results detected by eye or plate reader.

Old price: $274.00
$219.20  
You save: $54.80 (20%)
RT-LAMP-B-25
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Antigen Information

Protein Name & Synonyms:
Gene encoding for the Nucleocapsid (N) protein

Assay Format

Sensitivity:
25 viral RNA copies / µL
Compatible Sample Types:
  • Extracted RNA (e.g., from a throat swab)
Method of Detection:
Reverse transcription loop-mediated isothermal amplification (RT-LAMP)
Quantitative/Semi-Quantitative:
  • Qualitative (by eye) & Semi-Quantitative (with plate reader)

Product Specifications

Size:
25, 100, or 500 samples
Number of Targets Detected:
6 regions specific to the SARS-CoV-2 N gene. The regions selected for amplification do not share homology to nucleocapsid genes from any other coronaviruses.
Plate Reader Compatibility:
Absorbance at 570 nm and 650 nm

Introduction

This assay utilizes reverse transcription loop-mediated isothermal amplification (RT-LAMP), which amplifies SARS-CoV-2 RNA without the need for thermal cycling. This is accomplished using specialized primers that enable continuous amplification of nucleic acids at 60°C. RT-LAMP is faster than traditional RT-PCR with a colorimetric endpoint readout. Thus, detection of viral RNA can be performed by eye or with a plate reader capable of reading absorbance at 570 nm and 650 nm.

This kit uses six primer-probe sets specific to the SARS-CoV-2 gene encoding for the nucleocapsid protein. The regions selected for amplification do not share homology to nucleocapsid genes from any other coronaviruses.

Absence of viral RNA is represented by a purple color (570 nm)(see picture above). The presence of viral RNA is represented by a blue color (650 nm). An OD ratio of 650 nm / 570 more than 1.0 indicates that SARS-CoV-2 viral RNA is present.

Kit Components

Component 25 Tests 100 Tests 500 Tests
RayBio® COVID-19 Rapid Master Mix 375 μL 1.50 ml 1.50 ml x 5
SARS-CoV-2 Primer Mix 125 µl 500 µl 500 µl x 5
SARS-CoV-2 Synthetic Nucleocapsid RNA (1000 copies/µl) 10 μl 10 μl 10 μl x 5
Ultra-pure DNase- and RNase-free Water 1 ml 1 ml 1 ml x 5
Manual 1 1 1

This kit also has an option to include PCR tubes. 25 tests = 4 strips of 8 PCR tubes per strip. 100 tests = 13 strips of 8 PCR tubes per strip. 500 tests = 13 x 5 strips of 8 PCR tubes per strip.

Features

  • Rapid: Detect SARS-CoV-2 nucleic acid in 30 minutes
  • Affordable: No expensive PCR instrument required!
  • Easy to use: Read results by eye
  • Sensitive: Detects as few as 25 copies/µL of SARS-CoV-2 virus
  • Stable: Contains reagents to reduce DNA carry-over contamination. Also pH-independent.

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