This assay utilizes reverse transcription loop-mediated isothermal amplification (RT-LAMP), which amplifies SARS-CoV-2 RNA without the need for thermal cycling. This is accomplished using specialized primers that enable continuous amplification of nucleic acids at 60°C. RT-LAMP is faster than traditional RT-PCR with a colorimetric endpoint readout. Thus, detection of viral RNA can be performed by eye or with a plate reader capable of reading absorbance at 570 nm and 650 nm.
This kit uses six primer-probe sets specific to the SARS-CoV-2 gene encoding for the nucleocapsid protein. The regions selected for amplification do not share homology to nucleocapsid genes from any other coronaviruses.
Absence of viral RNA is represented by a purple color (570 nm)(see picture above). The presence of viral RNA is represented by a blue color (650 nm). An OD ratio of 650 nm / 570 more than 1.0 indicates that SARS-CoV-2 viral RNA is present.
|Component||25 Tests||100 Tests||500 Tests|
|RayBio® COVID-19 Rapid Master Mix||375 μL||1.50 ml||1.50 ml x 5|
|SARS-CoV-2 Primer Mix||125 µl||500 µl||500 µl x 5|
|SARS-CoV-2 Synthetic Nucleocapsid RNA (1000 copies/µl)||10 μl||10 μl||10 μl x 5|
|Ultra-pure DNase- and RNase-free Water||1 ml||1 ml||1 ml x 5|
This kit also has an option to include PCR tubes. 25 tests = 4 strips of 8 PCR tubes per strip. 100 tests = 13 strips of 8 PCR tubes per strip. 500 tests = 13 x 5 strips of 8 PCR tubes per strip.
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