COVID-19 Spike Variant-ACE2 Binding Assay Kit

The RayBio® COVID-19 Spike Variant-ACE2 binding assay kits are a rapid, simple, and sensitive method to characterize the binding affinity of the SARS-CoV-2 Spike (S) protein variants and the Angiotensin I Converting Enzyme 2 (ACE2) receptor complex in the presence of potential inhibitors.

Positive and negative controls for the Spike-ACE2 binding assays are available as an accessory item. Click here.

$510.00  
CoV-SM1ACE2-1
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Note:
The binding assay may be available in two different formats based on which protein is immobilized on the plate and which protein is added in solution (i.e. "query"). It is the researcher's responsibility to select the format for their study.

Antigen Information

Accession Number:
  • QHD43416
Gene Symbols:
  • ACE2
  • RBD
  • S
  • S-RBD
  • SRBD
Protein Name & Synonyms:
Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, Angiotensin-converting enzyme homolog, ACEH, Metalloprotease MPROT15, Processed angiotensin-converting enzyme 2, ACE2, Spike protein, S Protein, S1 Subunit, Host Cell Receptor Binding Domain (RBD)

Assay Format

Number of Targets Detected:
1
Species Detected:
  • Human
  • SARS-CoV-2
Compatible Sample Types:
  • Antibodies
  • Peptides
  • Plasma
  • Proteins
  • Serum
  • Small Molecules
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Application Notes

  • Evaluate inhibitor activity
  • Screen inhibitors of the SARS-CoV-2 Spike-ACE2 complex
  • Characterize antibody function
  • Vaccine development
  • Selection of patient serum for antibody therapy

Storage/Stability

Store kit at 4°C for up to 1 month from date of shipment.

Spike Variants

Multiple mutations of the wild type SARS-CoV-2 Spike protein (Accession no. QHD43416) have emerged since the COVID-19 pandemic began. RayBiotech offers inhibitor screening ELISA kits featuring the Spike RBD protein with mutations that appear in major SARS-CoV-2 variants of concern. The binding of the RBD to ACE2 enables viral entry.

SARS-CoV-2 Variant Classifications and Definitions

RayBiotech spike RBD mutant binding assays include:

N501Y mutant: present in Alpha, Beta, and Gamma variants (B.1.1.7, B.1.351, P.1)
Spike RBD protein with one amino acid mutation: N501Y.

E484K mutant: present in Beta and Gamma variants (B.1.351, P.1)
Spike RBD protein with one amino acid mutation: E484K.

Beta variant mutations (B.1.351)
Spike RBD protein with three amino acid mutations: K417N, E484K, N501Y.

Gamma variant mutations (P.1)
Spike RBD protein with three amino acid mutations: K417T, E484K, N501Y.

D614G mutant: present in Alpha, Beta, and Gamma variants (B.1.1.7, B.1.351, P.1)
Spike RBD protein with one amino acid mutation: D614G.

Alpha variant mutations (B.1.1.7)
Spike S1 protein mutations include a deletion in NTD at HV69-70 and two amino acid mutations in RBD: N501Y, D614G.

Check out our binding assay using the wild-type Spike variant here.

We used 4 different kits for assaying binding inhibition of patient serum from dried blood spot samples. The kits worked very well and enabled us to compare the binding inhibition for multiple samples, for multiple RBD types.

Research Foundation for SUNY - Polytechnic
We used this kit for evaluating patient blood serum for binding inhibition of the original RBD and RBD of the UK and S. African variants. The kit worked very well - we highly recommend!!!

SUNY Polytechnic Institute
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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