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Custom Human Dopamine beta-Hydroxylase ELISA Kit
RayBio® Human Dopamine beta-Hydroxylase ELISA Kit. 4 - 7 week lead time.
Product Description
Specifications
Size
1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species
Human
Protein Name / Synonyms
Dopamine beta-hydroxylase (EC 1.14.17.1) (Dopamine beta-monooxygenase) [Cleaved into: Soluble dopamine beta-hydroxylase]
Specificity
This ELISA antibody pair recognizes Human Dopamine beta-Hydroxylase.
Accession Number
P09172
Gene Symbols
DBH
Gene Id
1621
Quantitative/Semi-Quantitative
Quantitative
Solid Support
96-well Microplate
Method Of Detection
Colorimetric
Design Principle
Sandwich-based
Research Area
Hydroxylation, Post-Translational Modifications
Sensitivity
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Note
This ELISA uses an antibody pair and standard that have been validated for multiplex array products, but not for the 96-well plate ELISA format. Thus, this ELISA requires a 4 - 7 week development phase, which RayBiotech begins after receiving an order for the kit. After development, the kit will have passed RayBiotech's ELISA quality control tests, and will thereafter have a typical 1 - 2 day lead time for orders placed.
Estimated Lead Time
4-7 weeks
Risk-Free Guarantee
We offer a 100% guarantee on all ELISA Kits or C-Series Membrane Array.
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Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data
Kit Components
Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate
Other Materials Required
Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.
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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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