Name: Custom Human/Mouse Cleaved-Caspase-3 (D175) Cell-based ELISA Kit
SKU: CBEL-CASP3-D175-1
Availability: InStock

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	4-6 weeks
Species	Human, Mouse
Specificity	The antibody pair provided in this kit recognizes human/mouse cleaved-caspase-3 cleaved at site Aspartic Acid-175
Accession Number	P42574
Gene Id	836
Gene Symbols	CASP3, CPP32
Protein Name / Synonyms	Caspase-3 (CASP-3) (EC 3.4.22.56) (Apopain) (Cysteine protease CPP32) (CPP-32) (Protein Yama) (SREBP cleavage activity 1) (SCA-1) [Cleaved into: Caspase-3 subunit p17 Caspase-3 subunit p12]
Research Area	Apoptosis, Cancer, Post-Translational Modifications, Inflammation, Phosphorylation, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Product Features

Rapidly measure cleaved protein in adherent cell lines
Simultaneously measure cleaved protein and pan protein in one experiment (for normalization purpose)
No sample lysis is needed
Compatible with a standard ELISA plate reader

Application Notes

Kit Components

Uncoated 96-well Strip Microplate
Wash Buffers
Fixing Solution
Quneching Buffer
Blocking Buffer
Anti-cleaved Antibody
Anti-pan antibody
HRP-Conjugated Secondary Antibody
TMB One-Step Substrate
Stop Solution

Other Materials Required

Distilled or deionized water
100 ml and 1 liter graduated cylinders
Tubes to prepare sample dilutions
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
Benchtop rocker or shaker
Microplate reader capable of measuring absorbance at 450 nm

Protocol Outline

Seed 10,000-30,000 cells into each well and incubate overnight.
Apply various treatment, inhibitors or activators according to manufacturer's instructions.
Add 100 µl of Fixing Solution into each well and incubate for 20 min at RT with shaking.
Add 200 µl of prepared 1X Quenching Buffer and incubate 20 min at RT.
Add 200 µl of Blocking Solution and incubate for 1 h at 37°C.
Add 50 µl of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
Add 50 µl of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

Upon receipt, the kit should be stored at 20°C. Please use within 6 months from the date of shipment.

Product Features

Rapidly measure cleaved protein in adherent cell lines
Simultaneously measure cleaved protein and pan protein in one experiment (for normalization purpose)
No sample lysis is needed
Compatible with a standard ELISA plate reader

Application Notes

Kit Components

Uncoated 96-well Strip Microplate
Wash Buffers
Fixing Solution
Quneching Buffer
Blocking Buffer
Anti-cleaved Antibody
Anti-pan antibody
HRP-Conjugated Secondary Antibody
TMB One-Step Substrate
Stop Solution

Other Materials Required

Distilled or deionized water
100 ml and 1 liter graduated cylinders
Tubes to prepare sample dilutions
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
Benchtop rocker or shaker
Microplate reader capable of measuring absorbance at 450 nm

Protocol Outline

Seed 10,000-30,000 cells into each well and incubate overnight.
Apply various treatment, inhibitors or activators according to manufacturer's instructions.
Add 100 µl of Fixing Solution into each well and incubate for 20 min at RT with shaking.
Add 200 µl of prepared 1X Quenching Buffer and incubate 20 min at RT.
Add 200 µl of Blocking Solution and incubate for 1 h at 37°C.
Add 50 µl of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
Add 50 µl of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

Upon receipt, the kit should be stored at 20°C. Please use within 6 months from the date of shipment.

Custom Human/Mouse Cleaved-Caspase-3 (D175) Cell-based ELISA Kit

Product Description

Specifications

Product Features

Application Notes

Storage/Stability

Have this product in hand?

Custom Human/Mouse Cleaved-Caspase-3 (D175) Cell-based ELISA Kit

Custom Human/Mouse Cleaved-Caspase-3 (D175) Cell-based ELISA Kit

Product Description

Specifications

Product Features

Application Notes

Storage/Stability

Have this product in hand?

Custom Human/Mouse Cleaved-Caspase-3 (D175) Cell-based ELISA Kit

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Custom Human/Mouse Cleaved-Caspase-3 (D175) Cell-based ELISA Kit

Product Description

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Product Features

Application Notes

Storage/Stability

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Custom Human/Mouse Cleaved-Caspase-3 (D175) Cell-based ELISA Kit

Custom Human/Mouse Cleaved-Caspase-3 (D175) Cell-based ELISA Kit

Product Description

Specifications

Product Features

Application Notes

Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

Ask A Question

Custom Human/Mouse Cleaved-Caspase-3 (D175) Cell-based ELISA Kit

Services

Resources

About Us

Contact Us

Featured Products