1803

ADPSRKTYTL TDYLKNTYRL KLYSLRWISD HEYLYKQENN ILVFNAEYGN SSVFLENSTF DEFGHSINDY SISPDGQFIL LEYNYVKQWR HSYTASYDIY DLNKRQLITE ERIPNNTQWV TWSPVGHKLA YVWNNDIYVK IEPNLPSYRI TWTGKEDIIY NGITDWVYEE EVFSAYSALW WSPNGTFLAY AQFNDTEVPL IEYSFYSDES LQYPKTVRVP YPKAGAVNPT VKFFVVNTDS LSSVTNATSI QITAPASMLI GDHYLCDVTW ATQERISLQW LRRIQNYSVM DICDYDESSG RWNCLVARQH IEMSTTGWVG RFRPSEPHFT LDGNSFYKII SNEEGYRHIC YFQIDKKDCT FITKGTWEVI GIEALTSDYL YYISNEYKGM PGGRNLYKIQ LSDYTKVTCL SCELNPERCQ YYSVSFSKEA KYYQLRCSGP GLPLYTLHSS VNDKGLRVLE DNSALDKMLQ NVQMPSKKLD FIILNETKFW YQMILPPHFD KSKKYPLLLD VYAGPCSQKA DTVFRLNWAT YLASTENIIV ASFDGRGSGY QGDKIMHAIN RRLGTFEVED QIEAARQFSK MGFVDNKRIA IWGWSYGGYV TSMVLGSGSG VFKCGIAVAP VSRWEYYDSV YTERYMGLPT PEDNLDHYRN STVMSRAENF KQVEYLLIHG TADDNVHFQQ SAQISKALVD VGVDFQAMWY TDEDHGIASS TAHQHIYTHM SHFIKQCFSL PHHHHHH.

Greater than 95.0% as determined by Analysis by SDS-PAGE.

DPP4 is formulated in 20mM Tris-HCl buffer pH-8, 100mM NaCl, 1mM EDTA and 10% glycerol.

Assay Methods: Reaction buffer: 20mM Tris pH8.0, 0.1M NaCl, 1mM EDTA- Total reaction volume: 100 ul- Reaction temperature: 37°,C.
1. Add the reaction buffer to each well.
2. Add the 10 ul of 10mM substrate (Gly-Pro p-nitroanilide) to each well, Assay Methods: Reaction buffer: 20mM Tris pH8.0, 0.1M NaCl, 1mM EDTA- Total reaction volume: 100 ul- Reaction temperature: 37°,C.
1. Add the reaction buffer to each well.
2. Add the 10 ul of 10mM substrate (Gly-Pro p-nitroanilide) to each well.
3. Add the enzyme (DPP-4)
4. Incubate the 96 well plate at 37°,C.
5. Read the optical density at 405 nm. Optical density was measured at 405 nm after incubating enzyme solution with 1mM of p-nitroanilid as a substrate. 0ng DPP4= 0 OD at 405nm.
20ng DPP4= 0.075 OD at 405nm.
40ng DPP4= 0.125 OD at 405nm.
60ng DPP4= 0.2 OD at 405nm.
80ng DPP4= 0.275 OD at 405nm.
100ng DPP4= 0.35 OD at 405nm.