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DNA Topoisomerase-I 70kDa Recombinant Human
Catalog #: 228-12225
Product Description
Specifications
| Size | 20 µg, 1000 µg |
| Estimated Lead Time | 2-3 weeks |
| Species | Human |
| Accession Number | P11387 |
| Gene Id | 7150 |
| Protein Name / Synonyms | DNA topoisomerase 1, EC 5.99.1.2, DNA topoisomerase I, TOP1, Scl-70. |
| Expression System | Sf9 Insect Cells |
| Purity | Greater than 80% as determined by SDS-PAGE. |
| Formulation | TOP1 70kDa is supplied in 16mM HEPES buffer pH-8.0, 400mM NaCl, and 20% glycerol. |
| Recommended Applications | Western blot with Scleroderma patient sera or monoclonal anti-hexa-His-tag antibody. < Standard ELISA test (checkerboard analysis of positive/negative sera panels, including CDC international reference sera). (0.5-0.8 ug/ml depending on the type o, Western blot with Scleroderma patient sera or monoclonal anti-hexa-His-tag antibody. < Standard ELISA test (checkerboard analysis of positive/negative sera panels, including CDC international reference sera). (0.5-0.8 ug/ml depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination. Binds IgG-type human auto-antibodies. |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Description
Recombinant TOP1 70kDa protein is an enzyme fragment having a molecular mass of 69,199 Dalton (pH 9.4). TOP1 70kDa protein is fused to a hexa-histidine purification tag.
Stability & Storage
Storage/Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.