DNase Vial (D2)

Catalog #: 255-10035-1

A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 0.5 ml of Earle's Balanced Salt Solution (EBSS) or equivalent yields a solution of 2000 units…

Product Description

Specifications

Size	5 vi
Estimated Lead Time	1-2 weeks
Accession Number	P00639
Expression System	Bovine Pancreas
Molecular Weight (kDa)	29.1 kDa (Theoretical)
Activity	≥1,000 units per vial
Recommended Applications	DNA removal in primary cell isolation: decreases viscosity providing better yields DNA removal in bioprocessing applications Removing genomic DNA from RNA preparations prior to RT-PCR in vitro transcription Nick translation DNase footprinting Actin bindin, DNA removal in primary cell isolation: decreases viscosity providing better yields DNA removal in bioprocessing applications Removing genomic DNA from RNA preparations prior to RT-PCR in vitro transcription Nick translation DNase footprinting Actin binding UV crosslinking of proteins to nucleic acids Radioactive labeling
Research Area	Inflammation
Source	Bovine Pancreas
Shipping Type	Blue ice
Storage	2-8°C

Description

Other Information

Extinction Coefficient

36,750 cm-1 M-1 E1%,280 Equals 11.1

Isoelectric

5.08 (Theoretical)

CATH

Class: Alpha Beta Architecture: 4-Layer Sandwich Topology: Deoxyribonuclease I; Chain A

CAS Registry Number

C.A.S.: 9003-98-9

Storage Conditions

Store at 2-8°C.

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