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Dolphin IFN-gamma ELISA Kit
RayBio® Dolphin IFN-gamma ELISA Kit. 4 - 7 week lead time.
Product Description
Specifications
Size
1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species
Dolphin
Protein Name / Synonyms
Interferon gamma (IFN-gamma)
Specificity
This ELISA antibody pair recognizes Dolphin IFN-gamma.
Accession Number
Q9TV67
Gene Symbols
IFNG
Gene Id
101337436
Quantitative/Semi-Quantitative
Quantitative
Solid Support
96-well Microplate
Method Of Detection
Colorimetric
Design Principle
Sandwich-based
Sensitivity
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Note
This ELISA uses an antibody pair and standard that have been validated for multiplex array products, but not for the 96-well plate ELISA format. Thus, this ELISA requires a 4 - 7 week development phase, which RayBiotech begins after receiving an order for the kit. After development, the kit will have passed RayBiotech's ELISA quality control tests, and will thereafter have a typical 1 - 2 day lead time for orders placed.
Estimated Lead Time
4-7 weeks
Risk-Free Guarantee
We offer a 100% guarantee on all ELISA Kits or C-Series Membrane Array.
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$5 Amazon gift card in every kits purchased.
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data Kit Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate Other Materials Required
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis Protocol Outline
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately. Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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