Name: Dupilumab ELISA Kit
SKU: EAD-Dupi-1
Availability: InStock

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	3-5 business days
Sensitivity	1 pg/ml
Detection Range	1 pg/ml - 3000 pg/ml
Specificity	This ELISA antibody pair detects Dupilumab. Other targets not determined.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Protein Name / Synonyms	Dupilumab
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Design Principle	Sandwich-based
Method Of Detection	Colorimetric
Research Area	Antibody Drug Development, Drug Antibody (DA) Detection
Shipping Type	Blue ice
Storage	≤-20°C

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Lyophilized Standard
Biotinylated Detection Antibody
HRP-Streptavidin
Wash Buffer
Assay Diluent
TMB One-Step Substrate Reagent
Stop Solution

Other Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 µL to 1 mL volumes
Adjustable 1-25 mL pipettes for reagent preparation
100 mL and 1 liter graduated cylinders
Log-log graph paper or computer and software for ELISA data analysis
Absorbent paper
Tubes to prepare standard and sample dilutions
Distilled or deionized water
Shaker

Protocol Outline

Prepare all reagents, samples and standards as instructed.
Add 100 µL of each standard and sample to each well. Incubate 2 hours at room temperature or overnight at 4ºC with gentle shaking.
Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
Add 100 μL prepared HRP-Streptavidin solution. Incubate for 45 minutes at room temperature with gentle shaking.
Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Lyophilized Standard
Biotinylated Detection Antibody
HRP-Streptavidin
Wash Buffer
Assay Diluent
TMB One-Step Substrate Reagent
Stop Solution

Other Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 µL to 1 mL volumes
Adjustable 1-25 mL pipettes for reagent preparation
100 mL and 1 liter graduated cylinders
Log-log graph paper or computer and software for ELISA data analysis
Absorbent paper
Tubes to prepare standard and sample dilutions
Distilled or deionized water
Shaker

Protocol Outline

Prepare all reagents, samples and standards as instructed.
Add 100 µL of each standard and sample to each well. Incubate 2 hours at room temperature or overnight at 4ºC with gentle shaking.
Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
Add 100 μL prepared HRP-Streptavidin solution. Incubate for 45 minutes at room temperature with gentle shaking.
Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

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