The RayBio® E. coli Residual DNA Quantification Kit is a ready to use real-time PCR assay for the rapid and reliable detection of E. coli genomic DNA in biomanufacturing products.
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| Size | 100 tests |
| Estimated Lead Time | 3-5 business days |
| Species | E. coli |
| Sensitivity | 1.413 pg/mL |
| Specificity | The presence of genomic DNA did not affect the overall assay performance at either amount tested (10 and 1 ng/reaction). However, 10 ng of HEK293 or CHO gDNA per reaction caused a downward shift in Cq values, with an average decrease of 0.67 cycles for HEK293 gDNA and 0.51 cycles for CHO gDNA. No such shift was observed for 1 ng gDNA per reaction. |
| Compatible Sample Types | DNA |
| Quantitative/Semi-Quantitative | Quantitative |
| Method Of Detection | PCR |
| Research Area | Bioprocesses |
| Shipping Type | Dry ice (additional shipping fee) |
| Storage | -20°C |
RayBio® E. coli Residual DNA Quantification Kit is a ready to use real-time PCR assay for the rapid and reliable detection of E. coli genomic DNA in biomanufacturing products. This assay is highly sensitive (≤ 5 pg/mL), resistant to DNA fragmentation, and specific to E. coli, with no cross-reactivity to unrelated DNA products. An internal positive control, RNase P, is also detected in each reaction to monitor impact of potential PCR inhibitors.
| Component | Size / Qty |
|---|---|
| PCR Master Mix | 1 tube |
| Primer and Probe Mix | 1 tube |
| E. coli DNA Control | 1 tube |
| Dilution Buffer | 2 tubes |
Contact our technical support team for questions about compatibility: techsupport@raybiotech.com
The kit can be stored at -20°C for a period of 12 months prior to opening. After opening, the reagents are valid for at least 6 months if stored at -20°C (not exceeding 12 months since date of manufacture).
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Average limit of detection and limit of quantification across runs were 1.413 pg/mL and 2.867 pg/mL.
Average limit of detection and limit of quantification across runs were 1.413 pg/mL and 2.867 pg/mL.
